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Validation of DNA Sequences Using Mass Spectrometry Coupled with Nucleoside Mass Tagging
We present a mass spectrometry (MS)-based nucleoside-specific mass-tagging method to validate genomic DNA sequences containing ambiguities not resolved by gel electrophoresis. Selected types of (13)C/(15)N-labeled dNTPs are used in PCR amplification of target regions followed by matrix-assisted lase...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC186625/ https://ncbi.nlm.nih.gov/pubmed/12097352 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.221402 |
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