Impact of Competitive Inhibition and Sequence Variation upon the Sensitivity of Malaria PCR

Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were foun...

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Autores principales: Bialasiewicz, Seweryn, Whiley, David M., Nissen, Michael D., Sloots, Theo P.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2007
Materias:
Parasitology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1865911/
https://ncbi.nlm.nih.gov/pubmed/17329455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.02145-06
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