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Integration of Cot Analysis, DNA Cloning, and High-Throughput Sequencing Facilitates Genome Characterization and Gene Discovery

Cot-based sequence discovery represents a powerful means by which both low-copy and repetitive sequences can be selectively and efficiently fractionated, cloned, and characterized. Based upon the results of a Cot analysis, hydroxyapatite chromatography was used to fractionate sorghum (Sorghum bicolo...

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Autori principali: Peterson, Daniel G., Schulze, Stefan R., Sciara, Erica B., Lee, Scott A., Bowers, John E., Nagel, Alexander, Jiang, Ning, Tibbitts, Deanne C., Wessler, Susan R., Paterson, Andrew H.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC186575/
https://ncbi.nlm.nih.gov/pubmed/11997346
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.226102
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