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Effect of Osmotic Stress and Heat Shock in Recombinant Protein Overexpression and Crystallization
Overexpressed recombinant proteins in bacteria often tend to misfold and accumulate as soluble aggregates and/or inclusion bodies. A strategy for improving the level of expression of recombinant proteins in a soluble native form is to increase the cellular concentration of osmolytes or of chaperones...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2006
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1865119/ https://ncbi.nlm.nih.gov/pubmed/17126029 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2006.09.015 |
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