Direct polymerase chain reaction amplification of formalin‐fixed, paraffin‐wax‐embedded tissue after automated sequential laser microdissection and pressure catapulting

A robust method to facilitate rapid laser microdissection and pressure catapulting (LMPC) coupled with direct polymerase chain reaction (dPCR) to eliminate the need for extraction of DNA before a PCR‐based assay is described. This sequential LMPC–dPCR method is rapid and decreases the number of proc...

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Bibliographische Detailangaben
Hauptverfasser: O'Kane, S L, Garimella, V, Sivarajasingham, N, Drew, P J, Cawkwell, L
Format: Artigo
Sprache:Inglês
Veröffentlicht: BMJ Group 2007
Schlagworte:
Short Report
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1860634/
https://ncbi.nlm.nih.gov/pubmed/17264248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1136/jcp.2006.037275
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