Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens

BACKGROUND: Few reports of the utilization of an accurate, cost-effective means for measuring HPV oncogene transcripts have been published. Several papers have reported the use of relative quantitation or more expensive Taqman methods. Here, we report a method of absolute quantitative real-time PCR...

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Autores principales: Scheurer, Michael E, Dillon, Laura M, Chen, Zhuo, Follen, Michele, Adler-Storthz, Karen
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2007
Materias:
Methodology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1852093/
https://ncbi.nlm.nih.gov/pubmed/17407544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1750-9378-2-8
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