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Expression and purification of recombinant yeast Ada2/Ada3/Gcn5 and Piccolo NuA4 histone acetyltransferase complexes

Acetylation of histone tails by histone acetyltransferase (HAT) enzymes is a key post-translational modification of histones associated with transcriptionally active genes. Acetylation of the physiological nucleosome substrate is performed in cells by megadalton complexes such as SAGA and NuA4. To u...

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Detalhes bibliográficos
Main Authors: Barrios, Adam, Selleck, William, Hnatkovich, Brian, Kramer, Ryan, Sermwittayawong, Decha, Tan, Song
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1850971/
https://ncbi.nlm.nih.gov/pubmed/17309836
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2006.08.007
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