Purification and characterization of acidolysin, an acidic metalloprotease produced by Clostridium acetobutylicum ATCC 824.

Acidolysin an extracellular protease produced by Clostridium acetobutylicum ATCC 824 was purified to homogeneity by anion-exchange chromatography with a recovery of 91%. The enzyme was a monomeric protein with a molecular weight of 44,000 as estimated by sodium dodecyl sulfate-polyacrylamide gel ele...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Croux, C, Paquet, V, Goma, G, Soucaille, P
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC185045/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2082818/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.12.3634-3642.1990
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