Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer

BACKGROUND: To discover prostate cancer biomarkers, we profiled gene expression in benign and malignant cells laser capture microdissected (LCM) from prostate tissues and metastatic prostatic adenocarcinomas. Here we present methods developed, optimized, and validated to obtain high quality gene exp...

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Detalhes bibliográficos
Main Authors: Kube, Dagmar M, Savci-Heijink, Cemile D, Lamblin, Anne-Françoise, Kosari, Farhad, Vasmatzis, George, Cheville, John C, Connelly, Donald P, Klee, George G
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2007
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1847526/
https://ncbi.nlm.nih.gov/pubmed/17376245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-8-25
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