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Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer

BACKGROUND: To discover prostate cancer biomarkers, we profiled gene expression in benign and malignant cells laser capture microdissected (LCM) from prostate tissues and metastatic prostatic adenocarcinomas. Here we present methods developed, optimized, and validated to obtain high quality gene exp...

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Autors principals: Kube, Dagmar M, Savci-Heijink, Cemile D, Lamblin, Anne-Françoise, Kosari, Farhad, Vasmatzis, George, Cheville, John C, Connelly, Donald P, Klee, George G
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1847526/
https://ncbi.nlm.nih.gov/pubmed/17376245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-8-25
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