Cloning and characterization of the thymidylate synthase gene from Lactococcus lactis subsp. lactis.
The thymidylate synthase (thyA) gene has been isolated from Lactococcus lactis subsp. lactis. The cloned gene was strongly expressed in Escherichia coli both in vivo and in vitro (maxicells and cell-free transcription and translation systems) and complemented E. coli thyA mutants. DNA-DNA hybridizat...
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| Publié dans: | Appl Environ Microbiol |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1990
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184576/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2117882/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.7.2156-2163.1990 |
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