Cloning of the citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis and expression in Escherichia coli.

The citrate plasmid (Cit+ plasmid) from Lactococcus lactis subsp. lactis biovar diacetylactis was cloned into the EcoRI site of plasmid pUC18. This recombinant plasmid enabled Escherichia coli K-12 to transport and utilize citrate as a source of energy, indicating expression of the citrate permease...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Principais autores:	Sesma, F, Gardiol, D, de Ruiz Holgado, A P, de Mendoza, D
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1990
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184566/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2117878/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.7.2099-2103.1990
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