Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA

The advent of thermostable enzymes has led to great advances in molecular biology, such as the development of PCR and ligase chain reaction. However, isolation of naturally thermostable enzymes has been restricted to those existing in thermophylic bacteria. Here, we show that the disaccharide trehal...

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Main Authors: Carninci, Piero, Nishiyama, Yoko, Westover, Arthur, Itoh, Masayoshi, Nagaoka, Sumiharu, Sasaki, Nobuya, Okazaki, Yasushi, Muramatsu, Masami, Hayashizaki, Yoshihide
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 1998
Teme:
Biological Sciences
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC18452/
https://ncbi.nlm.nih.gov/pubmed/9435224
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