Thermostabilization and thermoactivation of thermolabile enzymes by trehalose and its application for the synthesis of full length cDNA

The advent of thermostable enzymes has led to great advances in molecular biology, such as the development of PCR and ligase chain reaction. However, isolation of naturally thermostable enzymes has been restricted to those existing in thermophylic bacteria. Here, we show that the disaccharide trehal...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Carninci, Piero, Nishiyama, Yoko, Westover, Arthur, Itoh, Masayoshi, Nagaoka, Sumiharu, Sasaki, Nobuya, Okazaki, Yasushi, Muramatsu, Masami, Hayashizaki, Yoshihide
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1998
Assuntos:	Biological Sciences
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC18452/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9435224/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.2.520
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