Polymerase chain reaction for detection of invasive Shigella flexneri in food.

The polymerase chain reaction (PCR) was used to amplify a 760-base-pair (bp) fragment with the 220-kbp invasive plasmids of enteroinvasive Escherichia coli, Shigella flexneri, Shigella dysenteriae, Shigella boydii, and Shigella sonnei as templates. This PCR product was easily detected by agarose gel...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Lampel, K A, Jagow, J A, Trucksess, M, Hill, W E
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1990
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184467/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2200336/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.6.1536-1540.1990
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