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Simple, Sensitive Zymogram Technique for Detection of Xylanase Activity in Polyacrylamide Gels

A method capable of detecting as little as 0.11 U of xylanase activity in polyacrylamide gels was developed. The method entails incubation of protein gels in contact with substrate gels containing unmodified xylan, followed by immersion of substrate gels in 95% ethanol. Resulting zymograms contain t...

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Autors principals: Royer, John C., Nakas, J. P.
Format: Artigo
Idioma:Inglês
Publicat: 1990
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC184463/
https://ncbi.nlm.nih.gov/pubmed/16348200
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