Isolation of behavioral mutants of Azospirillum brasilense by using Tn5 lacZ.

Tn5 lacZ mutants were generated with Azospirillum brasilense 7030 by mating that strain with Escherichia coli strains carrying suicide plasmid pCIB100 or pCIB110. Kanamycin-resistant Azospirillum colonies were obtained with a maximum frequency of 10(-6) per recipient cell. The potential of Tn5 lacZ...

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Publicado en:	Appl Environ Microbiol
Autores principales:	van Rhijn, P, Vanstockem, M, Vanderleyden, J, De Mot, R
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1990
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184333/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2160221/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.4.990-996.1990
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