Efficient DNA transformation of Bradyrhizobium japonicum by electroporation.
Intact cells of Bradyrhizobium japonicum USDA 110 were transformed with a 30-kilobase plasmid to efficiencies of 10(6) to 10(7) transformants per microgram by high-voltage electroporation. The technique was reliable and simple, with single colonies arising from transformed cells within 5 days of ant...
Guardado en:
| Publicado en: | Appl Environ Microbiol |
|---|---|
| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1990
|
| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184308/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2187405/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.56.4.833-836.1990 |
| Etiquetas: |
Sin Etiquetas, Sea el primero en etiquetar este registro!
|