Solubilization and renaturation of overexpressed aggregates of mutant tryptophan synthase alpha-subunits.

Certain Escherichia coli tryptophan synthase mutant alpha-subunits encoded from mutagenized trpA-containing plasmids were overexpressed as insoluble aggregates which were seen as large, intracellular inclusion bodies. The insoluble aggregates were solubilized to various degrees by several neutral, c...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Lim, W K, Smith-Somerville, H E, Hardman, J K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1989
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184261/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2547334/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.5.1106-1111.1989
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