Detection of Giardia cysts by using the polymerase chain reaction and distinguishing live from dead cysts.

A method was developed for the detection of Giardia cysts by using the polymerase chain reaction (PCR) and the giardin gene as the target. DNA amplification by PCR, using giardin DNA as the target, resulted in detection of both live and dead cysts. When giardin mRNA was used as the target, the abili...

Полное описание

Библиографические подробности

Опубликовано в::	Appl Environ Microbiol
Главные авторы:	Mahbubani, M H, Bej, A K, Perlin, M, Schaefer, F W, Jakubowski, W, Atlas, R M
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 1991
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183996/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1785923/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.12.3456-3461.1991
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