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Sensitivity Enhancement in (13)C Solid-State NMR of Protein Microcrystals by Use of Paramagnetic Metal Ions for Optimizing (1)H T(1) Relaxation
We discuss a simple approach to enhance sensitivity for (13)C high-resolution solid-state NMR for proteins in microcrystals by reducing (1)H T(1) relaxation times with paramagnetic relaxation reagents. It was shown that (1)H T(1) values can be reduced from 0.4-0.8 s to 60-70 ms for ubiquitin and lys...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2006
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1839830/ https://ncbi.nlm.nih.gov/pubmed/17126048 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmr.2006.10.012 |
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