A recombinase system facilitates cloning of expression cassettes in the ciliate Tetrahymena thermophila

BACKGROUND: Tetrahymena thermophila is one of the best characterized unicellular eukaryotes and its genome is sequenced in its entirety. However, the AT-richness of the genome and an unusual codon usage cause problems in cloning and expression of the ciliate DNA. To overcome these technical hiatuses...

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Bibliographic Details
Main Authors: Weide, Thomas, Bockau, Ulrike, Rave, Angelika, Herrmann, Lutz, Hartmann, Marcus WW
Format: Artigo
Language:Inglês
Published: BioMed Central 2007
Subjects:
Methodology Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1839094/
https://ncbi.nlm.nih.gov/pubmed/17328820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2180-7-12
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