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Studies of nucleotide binding to the catalytic sites of Escherichia coli βY331W-F(1)-ATPase using fluorescence quenching

Most studies of nucleotide binding to catalytic sites of Escherichia coli βY331W-F(1)-ATPase by the quenching of the βY331W fluorescence have been conducted in the presence of ≈20 mM sulfate. We find that, in the absence of sulfate, the nucleotide concentration dependence of fluorescence quenching i...

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Bibliographische Detailangaben
Hauptverfasser: Bulygin, Vladimir V., Milgrom, Yakov M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2007
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1838601/
https://ncbi.nlm.nih.gov/pubmed/17360523
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0700078104
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