Recovery of an integration shuttle vector from tandem repeats in Methanococcus maripaludis.

Transformation of Methanococcus maripaludis by using an integration vector, pKAS102, is described. Selection and subsequent growth at high concentrations of puromycin caused pKAS102 to develop tandem repeats within the genome. As a result, total DNA isolated from the transformant could be used to re...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Sandbeck, K A, Leigh, J A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183653/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1768149/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.9.2762-2763.1991
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