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Recovery of an integration shuttle vector from tandem repeats in Methanococcus maripaludis.

Transformation of Methanococcus maripaludis by using an integration vector, pKAS102, is described. Selection and subsequent growth at high concentrations of puromycin caused pKAS102 to develop tandem repeats within the genome. As a result, total DNA isolated from the transformant could be used to re...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Sandbeck, K A, Leigh, J A
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1991
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC183653/
https://ncbi.nlm.nih.gov/pubmed/1768149
Tagiau: Ychwanegu Tag
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