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Ultrasound Treatment for Harvesting an Aminopeptidase from Lactic Acid Bacteria and Quantitation of the Enzyme by Enzyme-Linked Immunosorbent Assays

Ultrasound treatment of Lactococcus lactis subsp. cremoris AM2 was optimized to release a maximum amount of intracellular aminopeptidase without modifying the antigenicity of the enzyme. The cells were sonicated three times for 30 s at 23 W. Antibodies produced against the aminopeptidase purified fr...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Boquien, C. Y., Nakache, F., Paraf, A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183552/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348536/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.8.2211-2216.1991
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