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Rapid confirmation of Listeria monocytogenes isolated from foods by a colony blot assay using a digoxigenin-labeled synthetic oligonucleotide probe.

An oligodeoxyribonucleotide probe based on the sequence of a 321-bp internal fragment of the msp gene encoding a major secreted polypeptide of Listeria monocytogenes was labeled with digoxigenin by using terminal deoxynucleotidyl transferase. The specificity of the digoxigenin-labeled probe was dete...

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Bibliografiske detaljer
Main Authors: Kim, C, Swaminathan, B, Cassaday, P K, Mayer, L W, Holloway, B P
Format: Artigo
Sprog:Inglês
Udgivet: 1991
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC183440/
https://ncbi.nlm.nih.gov/pubmed/1908203
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