Direct extraction of bacterial plasmids from food for polymerase chain reaction amplification.

In this report we describe a simple and rapid technique using DNA affinity columns that permits direct extraction of bacterial plasmids from a variety of foods for polymerase chain reaction amplification. The procedure was used to detect virulent enteroinvasive Escherichia coli in several artificial...

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Bibliographic Details

Published in:	Appl Environ Microbiol
Main Authors:	Andersen, M R, Omiecinski, C J
Format:	Artigo
Language:	Inglês
Published:	American Society for Microbiology (ASM) 1992
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183233/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1476448/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.12.4080-4082.1992
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