Rapid and sensitive method for detection of Shiga-like toxin-producing Escherichia coli in ground beef using the polymerase chain reaction.

A rapid and sensitive method for detection of Shiga-like toxin (SLT)-producing Escherichia coli (SLT-EC) with the polymerase chain reaction (PCR) is described. Two pairs of oligonucleotide primers homologous to SLTI and SLTII genes, respectively, were used in multiplex PCR assays. The first pair gen...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Gannon, V P, King, R K, Kim, J Y, Thomas, E J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC183186/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1476425/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.12.3809-3815.1992
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