Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction.
In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1992
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182998/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1444380/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.9.2717-2722.1992 |
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