Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction.

In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Picard, C, Ponsonnet, C, Paget, E, Nesme, X, Simonet, P
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1992
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182998/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1444380/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.58.9.2717-2722.1992
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