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Novel Method for Preparing Spheroplasts from Cells with an Internal Cellulosic Cell Wall

Protoplast and spheroplast preparations allow the transfer of macromolecules into cells and provide the basis for the generation of engineered organisms. Crypthecodinium cohnii cells harvested from polyethylene glycol-containing agar plates possessed significantly lower levels of cellulose in their...

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Detalhes bibliográficos
Main Authors: Kwok, Alvin C. M., Mak, Carmen C. M., Wong, Francis T. W., Wong, Joseph T. Y.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1828928/
https://ncbi.nlm.nih.gov/pubmed/17259549
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/EC.00301-06
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