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Localization of Ruminal Cellulolytic Bacteria on Plant Fibrous Materials as Determined by Fluorescence In Situ Hybridization and Real-Time PCR

To visualize and localize specific bacteria associated with plant materials, a new fluorescence in situ hybridization (FISH) protocol was established. By using this protocol, we successfully minimized the autofluorescence of orchard grass hay and detected rumen bacteria attached to the hay under a f...

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Detalhes bibliográficos
Main Authors: Shinkai, Takumi, Kobayashi, Yasuo
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1828787/
https://ncbi.nlm.nih.gov/pubmed/17209077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01896-06
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