Improved electroporation and cloning vector system for gram-positive bacteria.

A protocol for transformation of intact Enterococcus faecalis cells by electroporation was developed through a systematic examination of the effects of changes in various parameters, including (i) growth conditions; (ii) composition of the electroporation solution; (iii) electroporation conditions,...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Dunny, G M, Lee, L N, LeBlanc, D J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1991
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182867/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1905518/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.4.1194-1201.1991
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