Transformation of a Methylotrophic Bacterium, Methylobacterium extorquens, with a Broad-Host-Range Plasmid by Electroporation

An efficient method for the transformation of the methylotrophic bacterium Methylobacterium extorquens NR-2 with a broad-host-range plasmid, pLA2917, by electroporation was examined. Transformants of M. extorquens NR-2 expressing resistance to kanamycin were obtained after electric pulse. These tran...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Ueda, Shunsaku, Matsumoto, Seiji, Shimizu, Shoichi, Yamane, Tsuneo
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1991
Schlagworte:	Methods
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182823/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348470/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.4.924-926.1991
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