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Detection of Listeria monocytogenes by direct colony hybridization on hydrophobic grid-membrane filters by using a chromogen-labeled DNA probe.

A DNA probe specific for Listeria monocytogenes was isolated from a beta-hemolytic recombinant clone of an L. monocytogenes gene bank. It was labeled with horseradish peroxidase and used in a direct colony hybridization method on hydrophobic grid-membrane filters for the detection of the organism. F...

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Détails bibliographiques
Publié dans:Appl Environ Microbiol
Auteurs principaux: Peterkin, P I, Idziak, E S, Sharpe, A N
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1991
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182753/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1901711/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.2.586-591.1991
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