Secretion of TEM beta-lactamase with signal sequences isolated from the chromosome of Lactococcus lactis subsp. lactis.

With TEM beta-lactamase as a reporter gene, a set of expression-secretion-promoting fragments were isolated from the chromosome of Lactococcus lactis subsp. lactis. The fact that only translocated beta-lactamase renders cells resistant to ampicillin allowed direct ampicillin selection with an Escher...

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Bibliographic Details
Main Authors: Sibakov, M, Koivula, T, von Wright, A, Palva, I
Format: Artigo
Language:Inglês
Published: 1991
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC182716/
https://ncbi.nlm.nih.gov/pubmed/1901704
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