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Rapid Method To Characterize Lactococcal Bacteriophage Genomes

We present a rapid method to isolate and analyze bacteriophage DNA. Cells are infected and phage replication is allowed to proceed normally for 30 to 60 min. Prior to DNA packaging and cell bursts, the infected cells (1 ml) are harvested and lysed by using a combination of lysozyme and sodium dodecy...

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Detalhes bibliográficos
Main Authors: Hill, Colin, Massey, I. Joanna, Klaenhammer, Todd R.
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC182699/
https://ncbi.nlm.nih.gov/pubmed/16348396
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