Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils.

A modified protocol for DNA amplification by polymerase chain reaction (PCR) coupled with laser densitometric determination of the amount of PCR products, which allowed quantitation of target sequence numbers in soil extracts, was developed. The method was applied to monitor target loss during incub...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Romanowski, G, Lorenz, M G, Wackernagel, W
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182471/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8250566/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.10.3438-3446.1993
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