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Estimation of ruminal bacteriophage numbers by pulsed-field gel electrophoresis and laser densitometry.

To investigate phage activity in the rumen, a method for quantifying phage has been developed. By differential centrifugation and ultrafiltration, phage particles were separated and concentrated from ruminal fluid. Linear double-stranded DNA from this fraction containing predominantly tailed phage w...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Klieve, A V, Swain, R A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182272/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8357262/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.7.2299-2303.1993
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