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Detection of PCV2 DNA by SYBR Green I-based quantitative PCR

We developed an assay for the detection and quantitation of porcine circovirus type 2 (PCV2) with the SYBR Green I-based real-time PCR. The real-time PCR provides a broad dynamic range, detecting from 10(3) to 10(11) copies of DNA per reaction. No cross-reactions were found in specimens containing P...

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Detalhes bibliográficos
Main Authors: Yang, Zong-zhao, Habib, Mudasser, Shuai, Jiang-bing, Fang, Wei-huan
Formato: Artigo
Idioma:Inglês
Publicado em: Zhejiang University Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1810386/
https://ncbi.nlm.nih.gov/pubmed/17323427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1631/jzus.2007.B0162
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