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Analysis of the DNA-binding sequence specificity of the archaeal transcriptional regulator Ss-LrpB from Sulfolobus solfataricus by systematic mutagenesis and high resolution contact probing

To determine the sequence specificity of dimeric Ss-LrpB, a high resolution contact map was constructed and a saturation mutagenesis conducted on one half of the palindromic consensus box. Premodification binding interference indicates that Ss-LrpB establishes most of its tightest contacts with a si...

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Detalles Bibliográficos
Main Authors: Peeters, Eveline, Wartel, Carine, Maes, Dominique, Charlier, Daniel
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1802622/
https://ncbi.nlm.nih.gov/pubmed/17178749
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl1095
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