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Isolation of polysome-bound mRNA from solid tissues amenable for RT-PCR and profiling experiments

Using cell lines and primary cells, it has been shown that translation control plays a key role regulating gene expression during physiological and pathological conditions. The relevance of this type of regulation in vivo (tissues, organs) remains to be elucidated, due to the lack of an efficient me...

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Autores principales: Del Prete, M. Julieta, Vernal, Rolando, Dolznig, Helmut, Müllner, Ernst W., Garcia-Sanz, Jose A.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2007
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1800518/
https://ncbi.nlm.nih.gov/pubmed/17237355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.79407
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