Large-scale production of dsRNA and siRNA pools for RNA interference utilizing bacteriophage ϕ6 RNA-dependent RNA polymerase

The discovery of RNA interference (RNAi) has revolutionized biological research and has a huge potential for therapy. Since small double-stranded RNAs (dsRNAs) are required for various RNAi applications, there is a need for cost-effective methods for producing large quantities of high-quality dsRNA....

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Hlavní autoři: Aalto, Antti P., Sarin, L. Peter, van Dijk, Alberdina A., Saarma, Mart, Poranen, Minna M., Arumäe, Urmas, Bamford, Dennis H.
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2007
Témata:
Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1800515/
https://ncbi.nlm.nih.gov/pubmed/17237359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.348307
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