Real-Time PCR Assay To Detect Smallpox Virus

We developed a highly sensitive and specific assay for the rapid detection of smallpox virus DNA on both the Smart Cycler and LightCycler platforms. The assay is based on TaqMan chemistry with the orthopoxvirus hemagglutinin gene used as the target sequence. With genomic DNA purified from variola vi...

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Detalhes bibliográficos
Main Authors: Sofi Ibrahim, M., Kulesh, David A., Saleh, Sharron S., Damon, Inger K., Esposito, Joseph J., Schmaljohn, Alan L., Jahrling, Peter B.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC179856/
https://ncbi.nlm.nih.gov/pubmed/12904397
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.8.3835-3839.2003
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