Mutational analysis of the three cysteines and active-site aspartic acid 103 of ketosteroid isomerase from Pseudomonas putida biotype B.

In order to clarify the roles of three cysteines in ketosteroid isomerase (KSI) from Pseudomonas putida biotype B, each of the cysteine residues has been changed to a serine residue (C69S, C81S, and C97S) by site-directed mutagenesis. All cysteine mutations caused only a slight decrease in the k(cat...

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Publicado en:	J Bacteriol
Autores principales:	Kim, S W, Joo, S, Choi, G, Cho, H S, Oh, B H, Choi, K Y
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1997
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC179737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9401033/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.24.7742-7747.1997
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