Cloning and expression of a gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase from Erwinia cypripedii ATCC 29267 in Escherichia coli.

We have cloned the gene cluster encoding three subunits of membrane-bound gluconate dehydrogenase (GADH) from Erwinia cypripedii ATCC 29267 in Escherichia coli by performing a direct-expression assay. The positive clone converted D-gluconate to 2-keto-D-gluconate (2KDG) in the culture medium. Nucleo...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Yum, D Y, Lee, Y P, Pan, J G
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC179580/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9352901/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.21.6566-6572.1997
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