Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase.
The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia coli and purified. NifV is a homodimer that catalyzes the condensation of acetyl coenzyme A (acetyl-CoA) and alpha-ketoglutarate. Although alpha-ketoglutarate supports the highest level...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1997
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC179493/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9294461/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.18.5963-5966.1997 |
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