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A new Thermus-Escherichia coli shuttle integration vector system.

We established a Thermus thermophilus strain in which the pyrE gene (coding for orotate phosphoribosyltransferase of the pyrimidine biosynthetic pathway) was totally deleted. We also constructed an integration vector, which consisted of the Escherichia coli plasmid vector pBluescript and a 2.1-kb se...

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Main Authors: Tamakoshi, M, Uchida, M, Tanabe, K, Fukuyama, S, Yamagishi, A, Oshima, T
Formato: Artigo
Idioma:Inglês
Publicado: 1997
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC179328/
https://ncbi.nlm.nih.gov/pubmed/9244269
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