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Overexpression and characterization of a prolyl endopeptidase from the hyperthermophilic archaeon Pyrococcus furiosus.

The maltose-regulated mlr-2 gene from the hyperthermophilic archaeon Pyrococcus furiosus having homology to bacterial and eukaryal prolyl endopeptidase (PEPase) was cloned and overexpressed in Escherichia coli. Extracts from recombinant cells were capable of hydrolyzing the PEPase substrate benzylox...

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Detalhes bibliográficos
Main Authors: Harwood, V J, Denson, J D, Robinson-Bidle, K A, Schreier, H J
Formato: Artigo
Idioma:Inglês
Publicado em: 1997
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC179155/
https://ncbi.nlm.nih.gov/pubmed/9171407
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