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Lymphocytes from Enlarged Iliac Lymph Nodes as Fusion Partners for the Production of Monoclonal Antibodies after a Single Tail Base Immunization Attempt

A novel method of preparing hybridomas producing mouse monoclonal antibodies was ­established, called “the mouse iliac lymph node method”. Lymphocytes from enlarged iliac lymph nodes from mice injected intramuscularly at the tail base with an emulsion of antigen and Freund’s adjuvant were used for c...

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Hlavní autoři: Sado, Yoshikazu, Inoue, Satoko, Tomono, Yasuko, Omori, Hiroyuki
Médium: Artigo
Jazyk:Inglês
Vydáno: Japan Society of Histochemistry and Cytochemistry 2006
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On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1790974/
https://ncbi.nlm.nih.gov/pubmed/17327928
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1267/ahc.06001
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