Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate.

We have characterized the gene YOR347c of Saccharomyces cerevisiae and shown that it encodes a second functional pyruvate kinase isoenzyme, Pyk2p. Overexpression of the YOR347c/PYK2 gene on a multicopy vector restored growth on glucose of a yeast pyruvate kinase 1 (pyk1) mutant strain and could comp...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Boles, E, Schulte, F, Miosga, T, Freidel, K, Schlüter, E, Zimmermann, F K, Hollenberg, C P, Heinisch, J J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1997
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC179064/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9139918/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.9.2987-2993.1997
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