Linker mutagenesis of the Caulobacter crescentus S-layer protein: toward a definition of an N-terminal anchoring region and a C-terminal secretion signal and the potential for heterologous protein secretion.
Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4...
Shranjeno v:
| izdano v: | J Bacteriol |
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| Principais autores: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1997
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178737/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9006010/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.3.601-611.1997 |
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