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Linker mutagenesis of the Caulobacter crescentus S-layer protein: toward a definition of an N-terminal anchoring region and a C-terminal secretion signal and the potential for heterologous protein secretion.

Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4...

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Bibliografiset tiedot
Julkaisussa:J Bacteriol
Päätekijät: Bingle, W H, Nomellini, J F, Smit, J
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: American Society for Microbiology (ASM) 1997
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178737/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9006010/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.3.601-611.1997
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