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Purification of Vibrio cholerae fur and estimation of its intracellular abundance by antibody sandwich enzyme-linked immunosorbent assay.

The Vibrio cholerae fur gene was previously cloned and sequenced. A putative Fur box was identified in the divergent promoters of irgA, a virulence factor of V. cholerae, and irgB, a transcriptional activator of irgA. In this work, V. cholerae Fur was overexpressed in Escherichia coli and purified t...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: Watnick, P I, Eto, T, Takahashi, H, Calderwood, S B
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1997
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC178685/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8982004/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.179.1.243-247.1997
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